Referee Committee

 
Current Projects
2009-2010
2008-2009 Projects
2007-2008 Referee Projects

Referee Testing Procedures/Guidelines
Referee Project Database
(PDF)
AOSA Chair:
Ken Greger
Idaho State Seed Lab
Phone: 208-332-8635
Email: kgreger@idahoag.us
SCST Chair:
Jean Tolliver, RST
Monsanto Company
Phone: 815-264-8142
Email:
jean.h.tolliver@monsanto.com

Current Projects

Virtual Soybean Evaluation Referee
Field Corn Seedling Evaluation- Results & Answers posted 3/26/2010


2010 Virtual Soybean Evaluation Referee

Please fill out the following answer form that corresponds to the Virtual Soybean Evaluation Referee.  Although some of the seedlings may be border-line, please classify each seedling as either normal or abnormal.  Feel free to add comments as to why you classified the seedling that way and also if there was something in the image that made classification difficult.  All seedlings were grown on crepe cellulose paper (Kimpak™, VersaPak™).  A short survey follows the table.  Please send this form to Mike Stahr, Region 2 co-chairperson  Participants will qualify for one  continuing education point.   PLEASE RETURN RESULTS NO LATER THAN May 1, 2010.
Download Virtual Soybean Referee (PDF)    Download Answer Form (Word document)

Mike Stahr
128A Seed Science Center
Iowa State University
Ames, Iowa 50011
mgstahr@iastate.edu or 515-294-2014 (fax)


Referee Study on the Seedlings Evaluation of Field Corn (Zea mays)

Introduction:
This referee/ survey study is organized jointly by CSAAC and CFIA.  The referee is specially designed to obtain information intended to improve corn germination testing and to provide valuable feedback to the participating laboratories.  This is NOT intended to evaluate the proficiency of a participating lab, and multiple submissions from a participant lab are encouraged.

Objectives of the Study:

  • To promote precision, standardization, and uniformity among seed laboratories with regard to the evaluation of corn seedlings in the germination test.

  • To test the interpretation and adequacy of the existing Canadian Methods and Procedures for Seed Testing (M&P).

  • To provide data to be used as supporting evidence for future seed testing procedures and rule changes, such as harmonization with AOSA and ISTA seed testing procedures and rules.

  • To identify specific areas in corn germination test in which research is needed to promote uniformity among laboratories.

    Corn Seedling Evaluation Report      Answer PowerPoint
     


AOSA/SCST REFEREE RESEARCH PROJECTS 2009/2010

AOSA Committee Chair – Ken Greger         SCST Committee Chair – Jean Tolliver
kgreger@agri.idaho.gov                                 jean.h.tolliver@monsanto.com

Region I – Northwest
AOSA/SCST Regional Chair – Victor Shaul                 
vshaul@agr.wa.gov   

1.  Teff referee for introduction into the rules.
2.  Seed storage and viability of fine fescue.

Region II – Midwest
AOSA Regional Chair – Mike Stahr       SCST Regional Chair – Laura Carlson
mgstahr@iastate.edu                  laura.carlson@sgs.com

Region III – Northeast
AOSA Regional Chair – Chuck Boettinger         SCST Regional Chair – Johnanna Beuerlien
cboettinger@state.pa.us                                         johnanna@ohseed.org

1. Pelleted onion referee – extend test for optimum germination

Region IV – Southwest
AOSA Regional Chair – Annette Miller               SCST Regional Chair – Sue Alvarez
almiller@lamar.colostate.edu                                salvarez@cheerful.com

1. Pepper germ 1st count study – Donna Grubisic
2. Multiple seed units not listed specifically in the Rules – What do you do with them? Annette Miller

Region V – Southern
AOSA Regional Chair – Randy Bailey                SCST  Regional Chair – Amanda Patin
randy.bailey@ncmail.net                                     Amanda.Patin@sgs.com 

1. Teff referee – Germinaton temperature/evaluation

Region IV – Canada
AOSA Regional Chair – Janine Maruschak       SCST Regional Chair – Lisa Behnke
jmaruschak@inspection.gc.ca                             lisa@seedcheck.net  

1. Corn germination – comparison of alternate methods


AOSA/SCST REFEREE RESEARCH PROJECTS
2008/2009

AOSA Chair – Ken Greger                                SCST Chair – Jean Tolliver
kgreger@agri.idaho.gov                                 
jean.h.tolliver@monsanto.com

Region I – Northwest
AOSA Regional Chair – Victor Shaul                        SCST Regional Chair – Victor Shaul vshaul@agr.wa.gov                                                             vshaul@agr.wa.gov            

  • Pelleted Onion on Pleated Paper-germination-Don Ogawa
  • Florescence on ryegrass – Sabry Elias/Adriel Garay
  • Camelina Germ Referee – Sabry/Ariel

Region II – Midwest
AOSA Regional Chair – Mike Stahr                           SCST Regional Chair – Laura Carlson
mgstahr@iastate.edu                                                    laura.carlson@sgs.com

  • Soybean Virtual Evaluation Referee – Mike Stahr
  • Corn germination – Leaf < ½ length of coleoptile – Harold Armstrong
  • Germination referee-confectionary (sp) sunflowers –  BioDiagnostics

Region III – Northeast
AOSA Regional Chair – Chuck Boettinger             SCST Regional Chair – Norma Rossel
cboettinge@state.pa.us                                            nrossel@johnnyseeds.com

  • Pepper Referee – study of germination – Chuck Boettinger
  • Meadow Fescue ID Referee – Chuck Boettinger

Region IV – Southwest
AOSA Regional Chair – Ethan Waltermire              SCST Regional Chair – Sue Alvarez
csl@lamar.colostate.edu                            
               sue.alvarez@seminis.com      

  • Germination on Pleated Paper as an alternative method – sugar beets and table beets – Melissa Simpson/Annette Miller
  • Virtual Referee on Beta vulgaris – Melissa Simpson/Ethan Waltermire
  • Organic media for toxicity – Pelleted Sugar Beets & Coated Broccoli – Melissa Simpson/Gerardo Ramirez

Region V – Southern
AOSA Regional Chair – Randy Bailey                      SCST Regional Chair – Amanda Patin
randy.bailey@ncmail.net                                            Amanda.Patin@sgs.com  

  • Browntop Millet TZ vs Germ  – Aida Galarza

Region VI – Canada
AOSA Regional Chair – Janine Maruschak           SCST Regional Chair – Lisa Behnke
jmaruschak@inspection.gc.ca                                 lisa@seedcheck.net          

  • Asteraceae sp. ID – Lisa Behnke

AOSA/SCST REFEREE RESEARCH PROJECTS
2007/2008

AOSA Chair – Ken Greger                        SCST Chair – Jean Tolliver
   kgreger@agri.idaho.gov                                             jean.h.tolliver@monsanto.com

Region I – Northwest

AOSA/SCST Regional Chair – Victor Shaul vshaul@agr.wa.gov

  • Garden Pea temperature to 25°C – Mike Gerdes
  • 1/3 seed rule germination on thickspike wheatgrass or wildrye – Cindy Davis
  • Spinach Temperature @ 10°C or 20°C – Barbara Cleave
  • Florescence on ryegrass – Sabry Elias

Region II – Midwest

AOSA Regional Chair – Mike Stahr  mgstahr@iastate.edu                    
SCST Regional Chair – Laura Carlson 
 laurac@mwseed.com

  • Virtual Corn Seedling Referee – changes to Seedling Evaluation Handbook - Mike Stahr
  • Sunflower Referee – comparing the germination testing of different seed types of sunflower – Laura Carlson & Larry Prentice

Region III – Northeast

AOSA Regional Chair – Chuck Boettinger  cboettinge@state.pa.us             
SCST Regional Chair – Norma Rossel
 nrossel@johnnyseeds.com

  • Do hot peppers need to have a longer final count date
  • Methods for facilitating emergence of garden beans from seed coat for ease of seedling evaluation

Region IV – Southwest

AOSA Regional Chair – Ethan Waltermire  csl@lamar.colostate.edu                           
SCST Regional Chair – Sue Alvarez
  sue.alvarez@seminis.com       

  • Pelleted onion – Organic growing media vs standard germination (T) methods – Sue Alvarez & Ha Ung
  • Virtual Referee on Beta vulgaris – Ethan Waltermire
  • Sakata – Triploid watermelon germ – Guin Jenanyan& Olga Maseda

Region V – Southern

AOSA Regional Chair – Cindy Finneseth  Cindy.Finneseth@uky.edu       
SCST Regional Chair – Amanda Patin
amandap@mwseed.com           

  • Bromus Virtual & Real Seed ID Referee – Cindy Finneseth
  • Browntop Millet TZ vs Germ  – Paul Johnson

Region VI – Canada

AOSA Regional Chair – Janine Maruschak jmaruschak@inspection.gc.ca    
 SCST Regional Chair – Lisa Behnke   lisa@seedcheck.net      

  • Poacea ID – mixture of different sp – mix crop, weed, noxious, & foxtail

2006-2007 Projects:
AOSA Chair – Ken Greger                           SCST Chair – Jean Tolliver

Region I – Northwest
AOSA Regional Chair – Victor Shaul                        SCST Regional Chair –

  • Chickpea Germination– Extend germ days to 10 days – Victor Shaul
  • Ryegrass – Tall Fescue Calibrated blowing procedure
  • Red/White Wheat Cultivar Determination Survey

Region II – Midwest
AOSA Regional Chair – Mike Stahr              SCST Regional Chair – Laura Carlson

Region III – Northeast
AOSA Regional Chair – Chuck Boettinger   SCST Regional Chair – Norma Rossel

  • Bromus Seed ID & Cucurbitaceae Seed Id Referee
    • Digital Photo (same seeds as physical sample)
    • Seed Sample

Region IV – Southwest
AOSA Regional Chair – Ethan Waltermire   SCST Regional Chair – Sue Alvarez

  • Onion Pellet Fungicide-Insecticide Alternative Germ Methods
    • Tim Loeffler - Seminis
    • Jane Kohn – Incotec
  • Allium spp. Identification
    • Jim Effenberger, CDFA

Region V – Southern
AOSA Regional Chair – Cindy Finneseth     SCST Regional Chair – Amanda Patin

  • Continue Bromus ID Referee – Cindy Finneseth
    • Collaborate with Federal Lab
    • Improve images
  • Peanut Referee – Pat Stowe
    • Pathogen/fungicide

Region VI – Canada
AOSA Regional Chair – Janine Maruschak  SCST Regional Chair – Lisa Behnke

  • Flax germination, classification of normals and abnormals when cotyledon necrosis is present- Janine Maruschak.
  • Seed ID of various Poaceae species

2005-2006 Projects:
Region I- Northwest
Region II- Midwest
Region III- Northeast
Region IV- Southwest
Region V- South
Region VI- Canada
Open Project

2004-2005 Projects:
Region I- Northwest
Region II- Midwest
Region III- Northeast
Region IV- Southwest
Region V- South
Region VI- Canada

2003-2004 Projects:
Region I- Northwest
Region II- Midwest
Region III- Northeast
Region IV- Southwest
Region V- South
Region VI- Canada


REFEREE TESTING PROCEDURES

DefinitionA referee test is a specially designed test which is sent out to a number of seed laboratories in order to obtain information intended to improve seed testing and to provide valuable feedback to the participating laboratories.  Referee tests can be sent out either as independent sub samples (useful for purity, germination, biochemical tests, or other tests which would alter the sample) or as a “round-robin” sample, which is sent from one lab to the next (useful for seed identification referees).

Purpose:  Some important reasons for referee testing are listed here:

  • To promote precision, standardization, and uniformity among seed laboratories with regard to seed testing methods and interpretation in assessing seed quality.
  • To identify specific areas in seed testing in which research is needed to promote uniformity among laboratories.
  • To test the interpretation and adequacy of the existing AOSA Rules.
  • To test “new” methods which may prove to produce more uniform results than the existing AOSA Rules.
  • To determine if a new method is capable of producing uniform results for a species not yet found in the AOSA Rules.
  • To provide data to be used as supporting evidence for future AOSA Rules changes.
  • To serve as a seed identification training tool.

General Information on AOSA/SCST Referee Testing
Referee testing is coordinated by the Joint AOSA Referee/SCST Research committee, which has divided up the United States and Canada into six joint regions.  There are generally two projects per joint region per year.  The results of these referee projects are reported at the annual AOSA/SCST meeting in June of each year.  A data base of past referee projects is maintained by the joint AOSA/SCST Committee.  (Contact Deborah Meyer of the California Department of Food and Agriculture for the current list.)

Guidelines for Referee Testing

  • Define the purpose of the referee project; focus on a specific question in seed testing.
  • Check the data base of past referee projects and conduct a literature search before starting a referee project in order to determine whether similar projects have been previously conducted and to eliminate unnecessary duplication of projects.
  • Include the following with each referee sample:

–         Clearly defined purpose

–         Specific written instructions for method(s) to be used

–         Response sheet with clear instructions for recording data/information

–         Deadline date for returning data (time limit per lab for round-robin samples)

–         Address, phone number, FAX, and email address of project coordinator

  • When sending out potential herbarium specimens to laboratories for reference use, identification should be confirmed by taxonomic specialists.
  • It is beneficial to include educational materials with the referee to aid in the identification of seeds, seedling abnormalities, TZ staining patterns, etc.
  • When referee test results are compiled, data should be analyzed using appropriate statistical methods.  Assistance with statistical analysis may be provided by the AOSA Research Committee.
  • Project reports are due to the General Chairs of the Joint AOSA Referee/SCST Research Committee by April 30 prior to the annual meeting.  Project reports should include:  Title, Region, Purpose, Materials and Methods, Results, Discussion, Conclusions, Recommendations, Bibliography, Acknowledgments, and an Abstract.  The project coordinator is expected to provide 200 copies of the project report at the annual AOSA/SCST meeting in June.  In addition, the project coordinator gives a 10 minute oral report of the project at the open committee meeting.

2005-2006 Joint AOSA SCST Referee Projects
 

AOSA Chair:  Ken Greger                        SCST Chair: Jean TolliverContact:  kgreger@agri.state.id.us                          Contact: jean.h.tolliver@monsanto.com
208-332-8635                                             815-264-8142

Region I – Northwest
Chair:   Victor Shaul
Contact:        vshaul@agr.wa.gov
 
2005 Project:
Western Wheatgrass Detectable Endosperm in Comparison to One-Third the length of the Palea
 
2006 Projects:
Bean sand, towel germ
Chickpea, 7 to 10 day germ
Air velocity ryegrass

Region II – Midwest

AOSA Chair:  Mike Stahr                                      SCST Chair: Laura Carlson
Contact:  mgstahr@iastate.edu                             Contact:  laurac@mwseed.com

515-294-6826                                                          605-692-7611

2005 Project

  • Sorghum Substrata Comparison

2006 Project

  • Broken soybean seed held within the seed coat
  • Corn seedling evaluation

Region III – Northeast

AOSA Chair:  Ellen Chirco                                   SCST Chair:  Norma Rossel
Contact:  emc1@nysaes.cornell.edu                    Contact:  nrossel@johnnyseeds.com

315-787-2242                                                         207-861-3939 ext. 426

2005 Project

  • Joint Bromus ID

2006 Project:

  • Clarification of inert for damaged soybean seed
  • Cucurbit ID

Region IV – Southwest

AOSA Chair:  Annette Miller                                SCST Chair: Olga Maseda
Contact:  almiller@lamar.colostate.edu              Contact:  omaseda@sakata.com
970-495-3240                                                         408-782-5306

2005 Project

  • Germination requirement study for Atriplex confertifolia

2006 Project

  • Begonia – to describe and evaluate normal and abnormal seedlings from illustrations, photos, and live materials of two samples
    • Coordinators: Olga Maseda and Sue Alvarez
  • Lupinus – to germinate and TZ test two samples of Lupinus to examine consistency across labs
    • Coordinators: Terry Freeman, Denny Hall, and Annette Miller

Region V – Southern

AOSA Chair:  Cindy Finneseth                         SCST Chair: Nancy Meredith
Contact:  Cindy.Finneseth@uky.edu                Contact:  nancy.d.meredith@monsanto.com
410-841-5960                                                     870-673-8565

2006 Project

  • Pensacola Bahiagrass calibration sample
  • Bromus ID

Region VI – Canada

AOSA Chair:  Ken Allison                                                SCST Chair: Lisa Behnke
Contact:
kallison@inspection.gc.ca
(613) 759-1289
  

2005 Project

  • Treated Rapeseed Germination

2006 Project

  • Canadian Noxious Weed Seed
  • Field Bean Germination

Referee Project Open to Anyone:

Chair:              Rangegrass Committee, R. Denny Hall
Contact:          rdhall@uwyo.edu
307-754-4750

2006 Project

  • Purity referee on native Asteraceae for the purpose of developing a new pure seed unit definition

2004-2005 Joint AOSA SCST Referee Projects

Proposed Projects:
Region I – Northwest

AOSA Chair: Sabry Elias                                               SCST Chair: Cheryl Heilman
            Telephone: 541-737-4464                                   Telephone: 541-928-4014
            E-mail: sabry.elias@oscs.orst.edu                        E-mail: Cheryl@tangentseedlab.com
 
1) Tomato Germination/Treated vs Untreated
Contact: Pat Brownfield
Telephone: 208-465-8533
E-mail: pat.brownfield@syngentacom

2) Western Wheatgrass/Endosperm vs 1/3
Contact: Victor Shaul/Nancy Hartshorn
Telephone: 509-225-2630
E-mail: vshaul@agr.wa.gov

Region II – Midwest

AOSA Chair:    Dan Curry                                SCST Chair: Sharon Hanson-Gutormson
Telephone: 515-294-0117                                   Telephone: 605-692-7611          
E-mail: curry@iastate.edu                                   E-mail: sharonh@mwseed.com  

1) Sorghum Substrate Comparison
            Contact: Amanda Patin MWSS
            Telephone: 605-692-7611
            E-mail: amandap@mwseed.com
 
2) Corn Split Coleoptile
Contact: Sharon Gutormson                             Mike Stahr mgstahr@iastate.edu
Telephone: 605-692-7611
E-mail: sharonh@mwseed.com

Region III – Northeast

AOSA Chair:    Ellen Chirco                              SCST Chair:      Norma Rossel
            Telephone: 315-787-2242                                  Telephone: 207-437-9294 x 238 
            E-mail: emc1@nysaes.cornell.edu                     E-mail: norma@johnnyseeds.com
 
1) Bromus spp. ID
Contact: Ellen Chirco

2) TBA

Region IV – Southwest

Chair:   Annette Miller 
            Telephone: 970-495-3240
E-mail: almiller@lamar.colostate.edu        
 
1) Bromus ID
            Contact: Annette Miller
 
            2) Germ – Atriplex confertilfolia
            Contact: Terry Dunfield
            Telephone: 775-688-1182 ext: 279
            E-mail: Terry.Dunfield@Scotts.com

Region V – South

AOSA Chair: Lee Daughtry                                           SCST Chair:      Amanda Patin 
Telephone: 662-325-3992                                               Telephone: 605-692-7611
E-mail: Lee@mdac.state.ms.us                                     E-mail: amandap@mwseed.com
 
1) Germination of Partridge Pea
Contact: Lee Daughtry

2) Cotton Vigor Cool Test
Contact: Paul Johnson
Telephone: 662-686-5529
E-mail: pjohnson@stoneville.com 

Region VI – Canada

AOSA Chair:    Doug Ashton                                         CSAAC/SCST Chair: Cathy Breadner
Telephone: 613-759-1225                                               Telephone: 705-328-1648
E-mail: Ashton@inspection.gc.ca                                   E-mail: breadner@cdnseedlab.ca
 
1)Treated Rapeseed Germination
Contact: Doug Ashton

2) Birdsfoot Trefoil Seed Retrieval Method Comparison
Contact: Sharon Gane
Telephone: 306-862-4212
E-mail: Sharon.gane@swseed.se

Completed 2003-2004 SCST Research/AOSA Referee Projects

If you would like to receive a copy of these referee reports please contact Anita Hall, scst@twcny.rr.com Phone:607-256-3313

Region I – Northwest
Germination of Pelleted and Raw Onion Seeds

Region II – Midwest
Sorghum Substrata Comparison
Comparing Analysts Interpretation of New Rule Concerning Triticum spp., Wheat

Region III – Northeast
Malvaceae
Identification

Region IV – Southwest
2004 Artemesia tridentate Referee

Region VI – Canada
Identification of Seeds of Common and Durum Wheat
Treated Rapeseed Germination


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