Current
Projects
Virtual Soybean Evaluation Referee
Field Corn Seedling Evaluation- Results & Answers
posted 3/26/2010
2010
Virtual Soybean Evaluation Referee
Please fill out the following answer form that
corresponds to the Virtual Soybean Evaluation Referee. Although some of
the seedlings may be border-line, please classify each seedling as either
normal or abnormal. Feel free to add comments as to why you classified
the seedling that way and also if there was something in the image that
made classification difficult. All seedlings were grown on crepe
cellulose paper (Kimpak, VersaPak). A short survey follows the table.
Please send this form to Mike Stahr, Region 2 co-chairperson
Participants will qualify for one continuing education point.
PLEASE RETURN RESULTS NO LATER THAN May 1, 2010.
Download Virtual Soybean
Referee (PDF)
Download Answer
Form (Word document)
Mike Stahr
128A Seed Science Center
Iowa State University
Ames, Iowa 50011
mgstahr@iastate.edu or 515-294-2014 (fax)
Referee Study on the Seedlings Evaluation of Field Corn (Zea mays)
Introduction:
This referee/
survey study is organized jointly by CSAAC and CFIA.
The referee is specially designed to obtain information
intended to improve corn germination testing and to
provide valuable feedback to the participating
laboratories. This is NOT intended to
evaluate the proficiency of a participating lab, and
multiple submissions from a participant lab are
encouraged.
Objectives of the Study:
-
To promote precision, standardization, and
uniformity among seed laboratories with regard to the evaluation of corn
seedlings in the germination test.
-
To test the interpretation and adequacy of the existing Canadian Methods
and Procedures for Seed Testing (M&P).
-
To provide data to be used as supporting evidence for future seed
testing procedures and rule changes, such as harmonization with AOSA and
ISTA seed testing procedures and rules.
-
To identify specific areas in corn germination test in which research is
needed to promote uniformity among laboratories.
Corn Seedling
Evaluation Report
Answer
PowerPoint
AOSA/SCST REFEREE RESEARCH PROJECTS
2009/2010
AOSA Committee Chair Ken Greger SCST
Committee Chair Jean Tolliver
kgreger@agri.idaho.gov jean.h.tolliver@monsanto.com
Region I Northwest
AOSA/SCST Regional Chair Victor Shaul
vshaul@agr.wa.gov
1. Teff referee for
introduction into the rules.
2. Seed storage and viability of fine fescue.
Region II
Midwest
AOSA Regional Chair Mike Stahr SCST
Regional Chair Laura Carlson
mgstahr@iastate.edu laura.carlson@sgs.com
Region
III Northeast
AOSA Regional Chair Chuck Boettinger SCST
Regional Chair Johnanna Beuerlien
cboettinger@state.pa.us
johnanna@ohseed.org
1. Pelleted onion referee extend test for optimum germination
Region IV
Southwest
AOSA Regional Chair Annette Miller SCST
Regional Chair Sue Alvarez
almiller@lamar.colostate.edu
salvarez@cheerful.com
1. Pepper germ 1st count study Donna Grubisic
2. Multiple seed units not listed specifically in the Rules What do you
do with them? Annette Miller
Region V
Southern
AOSA Regional Chair Randy Bailey SCST
Regional Chair Amanda Patin
randy.bailey@ncmail.net
Amanda.Patin@sgs.com
1. Teff referee Germinaton temperature/evaluation
Region IV
Canada
AOSA Regional Chair Janine Maruschak SCST Regional
Chair Lisa Behnke
jmaruschak@inspection.gc.ca
lisa@seedcheck.net
1. Corn germination
comparison of alternate methods
AOSA/SCST REFEREE
RESEARCH PROJECTS
2008/2009
AOSA Chair Ken Greger
SCST Chair Jean Tolliver
kgreger@agri.idaho.gov
jean.h.tolliver@monsanto.com
Region
I Northwest
AOSA
Regional Chair Victor Shaul SCST Regional Chair
Victor Shaul
vshaul@agr.wa.gov
vshaul@agr.wa.gov
-
Pelleted Onion on Pleated Paper-germination-Don Ogawa
-
Florescence on ryegrass Sabry Elias/Adriel Garay
-
Camelina Germ Referee Sabry/Ariel
Region II Midwest
AOSA Regional Chair Mike Stahr
SCST Regional Chair Laura Carlson
mgstahr@iastate.edu
laura.carlson@sgs.com
-
Soybean Virtual Evaluation Referee Mike Stahr
-
Corn
germination Leaf < ½ length of coleoptile Harold Armstrong
-
Germination referee-confectionary (sp) sunflowers BioDiagnostics
Region III Northeast
AOSA Regional Chair Chuck Boettinger SCST
Regional Chair Norma Rossel
cboettinge@state.pa.us
nrossel@johnnyseeds.com
-
Pepper Referee study of germination Chuck Boettinger
-
Meadow Fescue ID Referee Chuck Boettinger
Region IV Southwest
AOSA Regional Chair Ethan Waltermire SCST
Regional Chair Sue Alvarez
csl@lamar.colostate.edu
sue.alvarez@seminis.com
-
Germination on Pleated Paper as an alternative method sugar beets and
table beets Melissa Simpson/Annette Miller
-
Virtual Referee on Beta vulgaris Melissa Simpson/Ethan Waltermire
-
Organic media for toxicity Pelleted Sugar Beets & Coated Broccoli
Melissa Simpson/Gerardo Ramirez
Region V Southern
AOSA Regional Chair Randy Bailey
SCST Regional Chair Amanda Patin
randy.bailey@ncmail.net
Amanda.Patin@sgs.com
-
Browntop Millet TZ vs Germ Aida Galarza
Region VI Canada
AOSA Regional Chair Janine Maruschak SCST
Regional Chair Lisa Behnke
jmaruschak@inspection.gc.ca
lisa@seedcheck.net
-
Asteraceae sp. ID Lisa Behnke
AOSA/SCST REFEREE
RESEARCH PROJECTS
2007/2008
AOSA Chair Ken
Greger
SCST Chair Jean Tolliver
kgreger@agri.idaho.gov
jean.h.tolliver@monsanto.com
Region I Northwest
AOSA/SCST Regional Chair Victor Shaul vshaul@agr.wa.gov
-
Garden Pea temperature to 25°C Mike Gerdes
- 1/3
seed rule germination on thickspike wheatgrass or
wildrye Cindy Davis
-
Spinach Temperature @ 10°C or 20°C Barbara Cleave
-
Florescence on ryegrass Sabry Elias
Region II Midwest
AOSA Regional Chair Mike Stahr
mgstahr@iastate.edu
SCST Regional Chair
Laura Carlson
laurac@mwseed.com
-
Virtual Corn Seedling Referee changes to Seedling
Evaluation Handbook - Mike Stahr
-
Sunflower Referee comparing the germination testing of
different seed types of sunflower Laura Carlson &
Larry Prentice
Region III Northeast
AOSA Regional Chair Chuck Boettinger
cboettinge@state.pa.us
SCST Regional Chair Norma Rossel
nrossel@johnnyseeds.com
- Do
hot peppers need to have a longer final count date
-
Methods for facilitating emergence of garden beans from
seed coat for ease of seedling evaluation
Region IV Southwest
AOSA Regional Chair Ethan Waltermire
csl@lamar.colostate.edu
SCST Regional Chair Sue Alvarez
sue.alvarez@seminis.com
-
Pelleted onion Organic growing media vs standard
germination (T) methods Sue Alvarez & Ha Ung
-
Virtual Referee on Beta vulgaris Ethan Waltermire
-
Sakata Triploid watermelon germ Guin Jenanyan& Olga
Maseda
Region V Southern
AOSA Regional Chair Cindy Finneseth
Cindy.Finneseth@uky.edu
SCST Regional Chair Amanda Patin
amandap@mwseed.com
-
Bromus Virtual & Real Seed ID Referee Cindy Finneseth
-
Browntop Millet TZ vs Germ Paul Johnson
Region VI Canada
AOSA Regional Chair Janine Maruschak jmaruschak@inspection.gc.ca
SCST
Regional Chair Lisa Behnke
lisa@seedcheck.net
-
Poacea ID mixture of different sp mix crop, weed,
noxious, & foxtail
2006-2007 Projects:
AOSA Chair Ken Greger SCST Chair Jean Tolliver
Region I Northwest
AOSA Regional Chair Victor Shaul SCST Regional
Chair
- Chickpea Germination Extend germ
days to 10 days Victor Shaul
- Ryegrass Tall Fescue Calibrated
blowing procedure
- Red/White Wheat Cultivar
Determination Survey
Region II Midwest
AOSA Regional Chair Mike Stahr SCST Regional Chair Laura
Carlson
Region III Northeast
AOSA Regional Chair Chuck Boettinger SCST Regional Chair Norma Rossel
- Bromus Seed ID & Cucurbitaceae Seed
Id Referee
- Digital Photo (same seeds as
physical sample)
- Seed Sample
Region IV Southwest
AOSA Regional Chair Ethan Waltermire SCST Regional Chair Sue Alvarez
- Onion Pellet Fungicide-Insecticide
Alternative Germ Methods
- Tim Loeffler - Seminis
- Jane Kohn Incotec
- Allium spp. Identification
Region V Southern
AOSA Regional Chair Cindy Finneseth SCST Regional Chair Amanda Patin
- Continue Bromus ID Referee Cindy
Finneseth
- Collaborate with Federal Lab
- Improve images
- Peanut Referee Pat Stowe
Region VI
Canada
AOSA Regional Chair Janine Maruschak SCST Regional Chair Lisa Behnke
- Flax germination, classification of
normals and abnormals when cotyledon necrosis is present- Janine
Maruschak.
- Seed ID of various Poaceae species
2005-2006 Projects:
Region I- Northwest
Region II- Midwest
Region III- Northeast
Region IV- Southwest
Region V- South
Region VI- Canada
Open Project
2004-2005 Projects:
Region I- Northwest
Region II- Midwest
Region III- Northeast
Region IV- Southwest
Region V- South
Region VI- Canada
2003-2004 Projects:
Region I- Northwest
Region II- Midwest
Region III- Northeast
Region IV- Southwest
Region V- South
Region VI- Canada
Definition: A referee test is a
specially designed test which is sent out to a number of seed laboratories
in order to obtain information intended to improve seed testing and to
provide valuable feedback to the participating laboratories. Referee tests
can be sent out either as independent sub samples (useful for purity,
germination, biochemical tests, or other tests which would alter the sample)
or as a round-robin sample, which is sent from one lab to the next (useful
for seed identification referees).
Purpose: Some important reasons for
referee testing are listed here:
- To promote
precision, standardization, and uniformity among seed laboratories with
regard to seed testing methods and interpretation in assessing seed
quality.
- To identify specific
areas in seed testing in which research is needed to promote uniformity
among laboratories.
- To test the
interpretation and adequacy of the existing AOSA Rules.
- To test new
methods which may prove to produce more uniform results than the existing
AOSA Rules.
- To determine if a
new method is capable of producing uniform results for a species not yet
found in the AOSA Rules.
- To provide data to
be used as supporting evidence for future AOSA Rules changes.
- To serve as a seed
identification training tool.
General Information on AOSA/SCST Referee Testing
Referee testing is coordinated by the Joint AOSA
Referee/SCST Research committee, which has divided up the United States and
Canada into six joint regions. There are generally two projects per joint
region per year. The results of these referee projects are reported at the
annual AOSA/SCST meeting in June of each year. A data base of past referee
projects is maintained by the joint AOSA/SCST Committee. (Contact Deborah
Meyer of the California Department of Food and Agriculture for the current
list.)
Guidelines for Referee Testing
- Define the purpose
of the referee project; focus on a specific question in seed testing.
- Check the data base
of past referee projects and conduct a literature search before starting a
referee project in order to determine whether similar projects have been
previously conducted and to eliminate unnecessary duplication of projects.
- Include the
following with each referee sample:
Clearly defined purpose
Specific written instructions for method(s) to be used
Response sheet with clear instructions for recording
data/information
Deadline date for returning data (time limit per lab for
round-robin samples)
Address, phone number, FAX, and email address of project
coordinator
- When sending out
potential herbarium specimens to laboratories for reference use,
identification should be confirmed by taxonomic specialists.
- It is beneficial to
include educational materials with the referee to aid in the
identification of seeds, seedling abnormalities, TZ staining patterns,
etc.
- When referee test
results are compiled, data should be analyzed using appropriate
statistical methods. Assistance with statistical analysis may be provided
by the AOSA Research Committee.
- Project reports are
due to the General Chairs of the Joint AOSA Referee/SCST Research
Committee by April 30 prior to the annual meeting. Project reports should
include: Title, Region, Purpose, Materials and Methods, Results,
Discussion, Conclusions, Recommendations, Bibliography, Acknowledgments,
and an Abstract. The project coordinator is expected to provide 200
copies of the project report at the annual AOSA/SCST meeting in June. In
addition, the project coordinator gives a 10 minute oral report of the
project at the open committee meeting.
2005-2006
Joint AOSA SCST Referee Projects
AOSA Chair: Ken
Greger
SCST Chair: Jean TolliverContact:
kgreger@agri.state.id.us Contact:
jean.h.tolliver@monsanto.com
208-332-8635
815-264-8142
- Region I Northwest
- Chair: Victor Shaul
- Contact:
vshaul@agr.wa.gov
-
- 2005 Project:
- Western Wheatgrass Detectable
Endosperm in Comparison to One-Third the length of the Palea
-
- 2006 Projects:
- Bean sand, towel germ
- Chickpea, 7 to 10 day germ
- Air velocity ryegrass
Region II Midwest
AOSA Chair: Mike
Stahr SCST Chair: Laura
Carlson
Contact:
mgstahr@iastate.edu Contact:
laurac@mwseed.com
515-294-6826
605-692-7611
2005 Project
-
Sorghum Substrata Comparison
2006 Project
-
Broken soybean seed held within the seed coat
- Corn
seedling evaluation
Region III
Northeast
AOSA Chair: Ellen
Chirco SCST Chair: Norma Rossel
Contact:
emc1@nysaes.cornell.edu Contact:
nrossel@johnnyseeds.com
315-787-2242
207-861-3939 ext. 426
2005 Project
2006 Project:
- Clarification of
inert for damaged soybean seed
- Cucurbit ID
Region IV Southwest
AOSA Chair: Annette
Miller
SCST Chair: Olga Maseda
Contact:
almiller@lamar.colostate.edu
Contact:
omaseda@sakata.com
970-495-3240
408-782-5306
2005 Project
- Germination
requirement study for Atriplex confertifolia
2006 Project
- Begonia to
describe and evaluate normal and abnormal seedlings from illustrations,
photos, and live materials of two samples
- Coordinators: Olga
Maseda and Sue Alvarez
- Lupinus to
germinate and TZ test two samples of Lupinus to examine consistency across
labs
- Coordinators:
Terry Freeman, Denny Hall, and Annette Miller
Region V Southern
AOSA Chair: Cindy
Finneseth SCST
Chair: Nancy Meredith
Contact:
Cindy.Finneseth@uky.edu Contact:
nancy.d.meredith@monsanto.com
410-841-5960
870-673-8565
2006 Project
-
Pensacola Bahiagrass calibration sample
-
Bromus ID
Region VI Canada
AOSA Chair: Ken
Allison
SCST Chair: Lisa Behnke
Contact:
kallison@inspection.gc.ca
(613) 759-1289
2005 Project
-
Treated Rapeseed Germination
2006 Project
-
Canadian Noxious Weed Seed
- Field
Bean Germination
Referee Project Open
to Anyone:
Chair:
Rangegrass Committee, R. Denny Hall
Contact:
rdhall@uwyo.edu
307-754-4750
2006 Project
-
Purity referee on native Asteraceae for the purpose of developing a new
pure seed unit definition
Proposed Projects:
Region I Northwest
- AOSA Chair: Sabry Elias
SCST Chair: Cheryl Heilman
Telephone:
541-737-4464 Telephone: 541-928-4014
E-mail:
sabry.elias@oscs.orst.edu
E-mail:
Cheryl@tangentseedlab.com
1) Tomato
Germination/Treated vs Untreated
Contact: Pat
Brownfield
Telephone:
208-465-8533
E-mail:
pat.brownfield@syngentacom
2) Western
Wheatgrass/Endosperm vs 1/3
Contact: Victor
Shaul/Nancy Hartshorn
Telephone:
509-225-2630
E-mail:
vshaul@agr.wa.gov
- AOSA Chair:
Dan Curry SCST Chair: Sharon Hanson-Gutormson
- Telephone:
515-294-0117 Telephone:
605-692-7611
- E-mail:
curry@iastate.edu E-mail:
sharonh@mwseed.com
1) Sorghum
Substrate Comparison
Contact: Amanda Patin MWSS
Telephone: 605-692-7611
E-mail:
amandap@mwseed.com
2) Corn
Split Coleoptile
Contact: Sharon
Gutormson Mike Stahr mgstahr@iastate.edu
Telephone:
605-692-7611
E-mail:
sharonh@mwseed.com
Region III Northeast
- AOSA Chair:
Ellen Chirco SCST Chair: Norma Rossel
- Telephone:
315-787-2242 Telephone: 207-437-9294 x
238
- E-mail: E-mail:
norma@johnnyseeds.com
1) Bromus
spp. ID
Contact: Ellen
Chirco
2) TBA
- Chair:
Annette Miller
- Telephone: 970-495-3240
- E-mail:
almiller@lamar.colostate.edu
1) Bromus
ID
Contact: Annette Miller
2)
Germ Atriplex confertilfolia
Contact: Terry Dunfield
Telephone: 775-688-1182 ext:
279
E-mail:
Region V South
- AOSA Chair: Lee Daughtry
SCST Chair: Amanda Patin
- Telephone:
662-325-3992 Telephone:
605-692-7611
- E-mail:
Lee@mdac.state.ms.us E-mail:
amandap@mwseed.com
1)
Germination of Partridge Pea
Contact: Lee
Daughtry
2) Cotton
Vigor Cool Test
Contact: Paul
Johnson
Telephone:
662-686-5529
E-mail:
pjohnson@stoneville.com
- AOSA Chair: Doug Ashton
CSAAC/SCST Chair: Cathy Breadner
- Telephone:
613-759-1225 Telephone:
705-328-1648
- E-mail:
Ashton@inspection.gc.ca E-mail:
breadner@cdnseedlab.ca
1)Treated
Rapeseed Germination
Contact: Doug
Ashton
2) Birdsfoot
Trefoil Seed Retrieval Method Comparison
Contact: Sharon
Gane
Telephone:
306-862-4212
E-mail:
Sharon.gane@swseed.se
Completed 2003-2004 SCST Research/AOSA Referee Projects
If you would like to receive a copy of these referee reports please contact
Anita Hall, scst@twcny.rr.com
Phone:607-256-3313
Region I Northwest
Germination of Pelleted and Raw Onion Seeds
Region II Midwest
Sorghum Substrata Comparison
Comparing Analysts Interpretation of New Rule Concerning Triticum spp., Wheat
Region III Northeast
Malvaceae
Identification
Region IV Southwest
2004 Artemesia tridentate Referee
Region VI Canada
Identification of Seeds of Common and Durum
Wheat
Treated Rapeseed Germination
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