Endophyte Testing Workshop
Statistics Workshop
Pathology/Genetic Testing Workshop
Tentative Agenda for the Endophyte Testing Workshop
May 31, 2009
The endophyte work session will be conducted in two parts one in the morning and one in the afternoon. The morning session will instruct participants how to detect endophyte in seed and stem tissue. Class participants will break out into pairs and work together as a team. Each team will receive two test kits one for seed analysis and one for grass tiller analysis. The class will go through the process step-by-step so all participants can get practical hands-on experience with endophyte detection. The session will be accompanied by a PowerPoint presentation to explain the significance of endophyte presence in grazing livestock, the conundrum of using endophyte-free seed in pastures, followed by a detailed description on how the immunochemical analysis works. The workshop will provide confidence to participants so they may run the analysis and provide insight towards interpretation of your results. Participants will have a chance to evaluate a new test protocol developed by Agrinostics Ltd. Co. and provide feedback as to which is a superior analysis.
The afternoon session will focus on alkaloid analysis in seeds and stem tissues for QA/QC of novel (non-toxic) endophyte detection.
Fee: $125 (includes lunch and breaks) Please note that attendance is limited to 25. In order for many labs to have a chance to participate we will limit enrollment to 2 per lab until April 15, 2009.
Morning Session:
| 7:45 am | Welcome, distribution of working materials for endophyte detection teams |
| 8:00 am | Getting started Preparing and placing seed/plant tissues on the test kit format for endophyte extractions and analysis. |
| 8:45 am | Beginning the endophyte reaction sequence how to prepare the extracted samples for analysis. (Blocking and adding mouse antibodies). (Brief PowerPoint presentation to describe what is happening during the first two steps. |
| 9:45 am | Continuing with analysis Washing and adding anti-mouse antibodies. (Continue with PowerPoint presentation) |
| 10:30 am | Break (refreshments included) |
| 11:00 am | Continuing with analysis Washing and Stacking the antibodies with chromophores (Continue with PowerPoint) |
| 11:45 am | The final step Washing and adding chromogen (color reagent) |
| 12:15 pm | Lunch Break |
| 1:15 pm | Divide into teams of four. Set up extraction of ergot alkaloids from seed tissues. (PowerPoint explanation on how to set up stem tissue for analysis) |
| 2:00 pm | Dispensing reagent extracted sample and antibody reagents for alkaloid analysis in seed (PowerPoint presentation continued) |
| 3:00 pm | Break |
| 4:00 pm | Washing and adding antimouse antibodies with chromophore (Continued PowerPoint presentation) |
| 5:00 pm | Washing and adding color reagent |
| 5:30 pm | Interpretation of results. Question/Answers |
| 6:00 pm | Soft Drinks, Wine and Cheese Compliments of Agrinostics Ltd. |
Statistics Workshop
June 1, 2009
Colorado State University
Experimental Design, Data Analysis, and Tolerances in Seed Testing
Fee:
$125 (includes lunch and breaks)
The workshop
will cover the following topics
● Quick overview of the principles of experimental design with practical examples Data Analysis: Analysis of variance (ANOVA); and mean separation
● Examples of using statistical packages in analyzing data
● Tolerances: What is tolerance? When to use it? How to use tolerances for purity tests, noxious weed seeds, germination, TZ, and moisture?
Instructors
Dr. Sabry Elias, AOSA Statistics Subcommittee Chair
Dr. Riad Baalbaki, AOSA Germination and Dormancy Subcommittee Chair
Pathology/Genetic
Technology Workshop
June 1, 2009
Location: Eurofins STA
1821 Vista View Dr., Longmont, CO 80504(303) 651-6417
Host: Eurofins STA Staff
Fee: $125 (includes transportation, lunch and breaks) Please note that attendance is limited to 25. In order for many labs to have a chance to participate we will limit enrollment to 2 per lab until April 15, 2009.
| 7:30am |
Meet in front of Fort Collins Hilton for transportation to Longmont |
| 8:30am | Welcome and Introductions John Mizicko & Darrell Maddox, |
| 8:45am |
National Seed Health System & Accredited - Lisa Shephard, Iowa State University Seed Science Center |
| 9:15am | Set up group rotations |
| 9:30am |
A-virus, B-mycology, C-bacteria, D-BFB |
| 10:45am | A-mycology, B-bacteria, C-BFB, D-virus |
| 12:00pm | Lunch |
| 1:00pm | A-bacteria, B-BFB, C-Virus, D-mycology |
| 2:15pm | A-BFB, B-virus, C-mycology, D-bacteria |
| 3:30pm | What is new in seed health testing - Karen McGuire, Envirologix |
| 4:00pm | The Accredited Seed Lab program- Perry Bohn, USDA |
| 4:30pm | Transportation from Eurofins STA back to Fort Collins Hilton |
| 5:30pm | Arrive back at Fort Collins Hilton |
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